Age-related macular degeneration

22 September 2017 in In Vivo

Choroidal neovascularization model (CNV)

CNV is a commonly used and popular model to mimic the wet form of age-related macular degeneration (AMD). The model is induced by laser photocoagulation of Bruchs membrane. The pathological cascade includes inflammation, angiogenesis and proteolysis (Grossniklaus et al., 2010).

Species: mouse and rat
Method of induction: 532-nm diode laser
Follow-up period: typically up to 2 weeks
Typical group size: ≥10 animals
Route of compound administration: topical, intravitreal, subretinal, systemic
Read-outs:
• In vivo imaging (fundoscopy, fluorescein angiography, optical coherence tomography (OCT))
• Morphological assessment (histology, IHC, EM, stereology)
• Molecular biology (ELISA, Western blotting, qPCR)

Reference:

Grossniklaus HE, Kang SJ, Berglin L. Animal models of choroidal and retinal neovascularization. Prog Retin Eye Res. 2010 Nov;29(6):500-19. doi: 10.1016/j.preteyeres.2010.05.003.PubMed PMID: 20488255

 

Light damage model

Species: mouse (Balb/C)
Method of induction: Acute or chronic exposure to cool white LED light (2,500-10,000 lux)
Follow-up period: 1-5 weeks
Typical group size: ≥10 animals
Route of compound administration: topical, intravitreal, subretinal, systemic
Read-outs:
• In vivo imaging (optical coherence tomography (OCT))
• In vivo functional assessment: a) electroretinography (ERG), b) visual evoked potential measurements (VEP)
• Morphological assessment (histology, IHC, EM, stereology)
• Molecular biology (ELISA, Western blotting, qPCR)

Reference:

Kalesnykas G, Ragauskas S, Kaja S, Tanila H, Leinonen HO. Age-related differences in light sensitivity in BALB/c mice. ARVO2016, abstract no.: 5004.

Our previous expertise on ocular vascular pathology:

1.    Kinnunen K, Heinonen SE, Kalesnykas G, Laidinen S, Uusitalo-Järvinen H, Uusitalo H, Ylä-Herttuala S. LDLR-/-ApoB100/100 mice with insulin-like growth factor II overexpression reveal a novel form of retinopathy with photoreceptor atrophy and altered morphology of the retina. Mol Vis. 2013 Aug 4;19:1723-33. PubMed PMID: 23922490.

2.    Viita H, Kinnunen K, Eriksson E, Lähteenvuo J, Babu M, Kalesnykas G, Heikura T, Laidinen S, Takalo T, Ylä-Herttuala S. Intravitreal adenoviral 15-lipoxygenase-1 gene transfer prevents vascular endothelial growth factor A-induced neovascularization in rabbit eyes. Hum Gene Ther. 2009 Dec;20(12):1679-86. doi: 10.1089/hum.2009.069. PubMed PMID: 19694557.

3.    Kinnunen K, Kalesnykas G, Mähönen AJ, Laidinen S, Holma L, Heikura T, Airenne K, Uusitalo H, Ylä-Herttuala S. Baculovirus is an efficient vector for the transduction of the eye: comparison of baculovirus- and adenovirus-mediated intravitreal vascular endothelial growth factor D gene transfer in the rabbit eye. J Gene Med. 2009 May;11(5):382-9. doi: 10.1002/jgm.1311. PubMed PMID: 19263462.

For more information contact us directly.