Streptozotocin (STZ) is a N-nitro derivative of glucosamine that is toxic to the insulin-producing pancreatic beta-cells. STZ-induced diabetic retinopathy model represents a popular and highly reproducible preclinical model to mimic type I diabetes mellitus in humans. However, this model mimics only very early phase of diabetic retinopathy without prominent vascular changes in the retina.
Method of Induction: Streptozotocin injection (ip or iv)
Induction success rate: 80-90%
Follow-up period: typically 3-6 months
Route of compound administration: topical, intravitreal, subretinal, systemic
• In vivo imaging (fundoscopy, fluorescein angiography, optical coherence tomography (OCT))
• In vivo functional assessment: electroretinography (ERG)
• Morphological assessment (histology, IHC, EM, stereology)
• Molecular biology (ELISA, Western blotting, qPCR)
Haapaniemi AM, Nedorubov AA, Kalesnykas G, Shram SI. Diabetes in rats does not cause significant retinal degeneration, but upregulates heat shock protein response. Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5400.
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