Diabetic retinopathy

27 July 2017 in In Vivo

Streptozotocin (STZ) is a N-nitro derivative of glucosamine that is toxic to the insulin-producing pancreatic beta-cells. STZ-induced diabetic retinopathy model represents a popular and highly reproducible preclinical model to mimic type I diabetes mellitus in humans. However, this model mimics only very early phase of diabetic retinopathy without prominent vascular changes in the retina.

Species: Rats
Method of Induction: Streptozotocin injection (ip or iv)
Induction success rate: 80-90%
Follow-up period: typically 3-6 months
Route of compound administration: topical, intravitreal, subretinal, systemic
Read-outs:
• In vivo imaging (fundoscopy, fluorescein angiography, optical coherence tomography (OCT))
• In vivo functional assessment: electroretinography (ERG)
• Morphological assessment (histology, IHC, EM, stereology)
• Molecular biology (ELISA, Western blotting, qPCR)

Our reference:

Haapaniemi AM, Nedorubov AA, Kalesnykas G, Shram SI. Diabetes in rats does not cause significant retinal degeneration, but upregulates heat shock protein response. Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5400.

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