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Category: Neuroprotection
Animals used for research:  Mouse, Rat 

Organotypic retinal tissue ex vivo provides a simplified model to study retinal cell degeneration and screen promising therapeutics under strictly controlled environmental conditions.1

Indications: Neuroprotection, retinitis pigmentosa

SUMMARY

Animal species

Mice, rats

Follow-up period

Up to 14 days

Route of compound administration

In the serum-free media

Read-outs
  • Ex vivo functional assessment;
  • Morphological assessment;
  • Molecular biology (ELISA, Western blotting, qPCR).
Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

simplified overview of methodologies used for ex vivo retinal explant studies

Figure 1.  An overview of employed methodology to study ex vivo retinal explants. 1) Evaluation of retinal thickness and glial/microglial activation from retinal sections; 2) Estimation of the total number of neurons/astrocytes/microglial cells using stereology; 3) Functional evaluation of retinal tissue using multi-electrode array system.

SELECTED PUBLICATIONS

  • Nymark S, Ragauskas S, Savolainen V, Holme A, Hyttinen JAK, Kalesnykas G. Morphological and functional characterization of ex vivo retinal explants from adult mice. Investigative Ophthalmology & Visual Science, 2014, April, Vol. 55, 2377.

REFERENCES

  1. Bull ND, Johnson TV, Welsapar G, DeKorver NW, Tomarev SI, Martin KR. Use of an adult rat retinal explant model for screening of potential retinal ganglion cell neuroprotective therapies. Invest Ophthalmol Vis Sci. 2011 May 17;52(6):3309-20. doi:10.1167/iovs.10-6873.

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