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Category: Angiogenesis

To test the anti-angiogenic potential of an NCE/compound in human umbilical vein endothelial cells (HUVEC) in vitro.



Human umbilical vein endothelial cells (HUVEC)


15-well µ-Slide Angiogenesis (Ibidi GmbH, Germany)

Angiogenic inducers and positive control

Inducers: incl. angiogenin and VEGF;
Positive control: sulforaphane.


ImageJ or Wimasis (Khoo et al., 2011)

Please contact us, if you would like to receive more information:


Human umbilical vein endothelial cells (HUVECs)

Assessment of anti-angiogenic properties of sulforaphane after an induction of angiogenesis using angiogenic and VEGF


  • Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB. Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis. Stem Cell Rev. 2006;2(2):93-102. Review. PubMed PMID: 17237547.
  • Cao Y, Gong Y, Liu L, Zhou Y, Fang X, Zhang C, Li Y, Li J. The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review. J Appl Toxicol. 2017 Dec;37(12):1359-1369. doi: 10.1002/jat.3470. Epub 2017 Apr 6. Review. PubMed PMID: 28383141.


  • Khoo CP, Micklem K, Watt SM. A comparison of methods for quantifying angiogenesis in the Matrigel assay in vitro. Tissue Eng Part C Methods. 2011 Sep;17(9):895-906. doi: 10.1089/ten.TEC.2011.0150. Epub 2011 Jun 8. PubMed PMID: 21517696;

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