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Category: Angiogenesis

To test the anti-angiogenic potential of an NCE/compound in human umbilical vein endothelial cells (HUVEC) in vitro.

SUMMARY

Cells

Human umbilical vein endothelial cells (HUVEC)

Format

15-well µ-Slide Angiogenesis (Ibidi GmbH, Germany)

Angiogenic inducers and positive control

Inducers: incl. angiogenin and VEGF;
Positive control: sulforaphane.

Analysis

ImageJ or Wimasis (Khoo et al., 2011)

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IMAGES

Human umbilical vein endothelial cells (HUVECs)

Assessment of anti-angiogenic properties of sulforaphane after an induction of angiogenesis using angiogenic and VEGF

SELECTED PUBLICATIONS

  • Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB. Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis. Stem Cell Rev. 2006;2(2):93-102. Review. PubMed PMID: 17237547.
  • Cao Y, Gong Y, Liu L, Zhou Y, Fang X, Zhang C, Li Y, Li J. The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review. J Appl Toxicol. 2017 Dec;37(12):1359-1369. doi: 10.1002/jat.3470. Epub 2017 Apr 6. Review. PubMed PMID: 28383141.

REFERENCES

  • Khoo CP, Micklem K, Watt SM. A comparison of methods for quantifying angiogenesis in the Matrigel assay in vitro. Tissue Eng Part C Methods. 2011 Sep;17(9):895-906. doi: 10.1089/ten.TEC.2011.0150. Epub 2011 Jun 8. PubMed PMID: 21517696;

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