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Category: Neuroprotection / glioprotection

An assessment and prediction of study compound-induced neuroprotection / toxicity on the rat primary cortical neurons in vitro.

SUMMARY

Cells

Rat primary cortical neurons

Oxidative stress inducer

tert-butyl hydroperoxide (tBHP) at concentrations 0-40 µM

Positive control

(±)-6-Hydroxy-2,5,7,8- tetramethylchromane-2-carboxylic acid (Trolox) 1000 µM

Indicator and detection

Indicator: resazurin (7-Hydroxy-3H-phenoxazin-3-one-10-oxide) dye is irreversibly reduced to highly fluorescent metabolite resorufin (7-Hydroxy-3H-phenoxazin-3-one);

 

Detection: after 2 h exposure to resazurin, the amount of resorufin is measured at 560 nm (ex), 590 nm (em) by using Cytation 3 multi-mode reader (BioTek Instruments, Winooski, VT, USA).

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IMAGES

Rat primary cortical neurons

Immunostaining of the rat primary cortical neurons (in red) counterstained with cellular nuclear marker DAPI (in blue).

SELECTED PUBLICATIONS

  • Kaja S, Duncan RS, Longoria S, Hilgenberg JD, Payne AJ, Desai NM, Parikh RA, Burroughs SL, Gregg EV, Goad DL, Koulen P. Novel mechanism of increased Ca2+ release following oxidative stress in neuronal cells involves type 2 inositol-1,4,5-trisphosphate receptors. Neuroscience. 2011,175:281-291. doi: 10.1016/j.neuroscience.2010.11.010.

REFERENCES

  1. Burroughs SL, Duncan RS, Rayudu P, Kandula P, Payne AJ, Clark JL, Koulen P, Kaja S. Plate reader-based assays for measuring cell viability, neuroprotection and calcium in primary neuronal cultures. J Neurosci Methods. 2012,203(1):141-145. doi: 10.1016/j.jneumeth.2011.09.007.

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