Custom biomarker projects
Custom biomarker projects
Category: Custom R&D models
Experimentica’s in vitro team combines decade’s of experience in cellular models, biomarker discovery and imaging modalities to provide you with state-of-the-art biomarker identification and detection in in vitro and in vivo models.
We look forward to discussing your specific project with you and designing a dedicated experimental strategy, conducting any needed pilot experiments, and assisting you with the data analysis.
Our team of scientists has a strong track-record in biomarker detection and imaging modalities in the ophthalmic and neuroscience space and access to state-of-the-art equipment, so you can always be assured that your project will be conducted to your specifications.
SUMMARY
Models
Cell lines, primary cells, tissue and biopsy samples, in vivo models.
Cell lines, primary cells, tissue and biopsy samples, in vivo models.
In vitro insults
TBD
TBD
Read-outs
- Multiplex immunoassays (Luminex);
- ELISA;
- Quantitative PCR;
- High resolution confocal microscopy;
- Electron microscopy;
- Whole-cell patch-clamp electrophysiology.
- Multiplex immunoassays (Luminex);
- ELISA;
- Quantitative PCR;
- High resolution confocal microscopy;
- Electron microscopy;
- Whole-cell patch-clamp electrophysiology.
Examples
The following is a non-comprehensive list of the type of custom project we routinely conduct:
- Identification of target gene and/or protein expression in specific tissues or regions;
- Cytokine analysis in tissues, e.g. corneal tissue from our dry eye disease model;
- Changes of protein expression and distribution in animals models for ophthalmic disease, aging, neurodegeneration and inflammation by confocal microscopy;
- Determination of barrier integrity using imaging modalities (e.g. blood-retinal barrier, blood-brain barrier).
The following is a non-comprehensive list of the type of custom project we routinely conduct:
- Identification of target gene and/or protein expression in specific tissues or regions;
- Cytokine analysis in tissues, e.g. corneal tissue from our dry eye disease model;
- Changes of protein expression and distribution in animals models for ophthalmic disease, aging, neurodegeneration and inflammation by confocal microscopy;
- Determination of barrier integrity using imaging modalities (e.g. blood-retinal barrier, blood-brain barrier).
Please contact us, if you would like to receive more information: info@experimentica.com
IMAGES
Representative examples from primary rat optic nerve head astrocytes
Oxidative stress and cell viability in primary optic nerve head astrocytes
SELECTED PUBLICATIONS
- Vaajanen A, Kalesnykas G, Vapaatalo H, Uusitalo H. The expression of Mas-receptor of the renin-angiotensin system in the human eye. Graefes Arch Clin Exp Ophthalmol. 2015 Jul;253(7):1053-9.
- Vaajanen A, Kalesnykas G, Vapaatalo H, Uusitalo H. The expression of Mas-receptor of the renin-angiotensin system in the human eye. Graefes Arch Clin Exp Ophthalmol. 2015 Jul;253(7):1053-9.
REFERENCES
- Kaja S, Payne AJ, Naumchuk Y, Levy D, Zaidi DH, Altman AM, Nawazish S, Ghuman JK, Gerdes BC, Moore MA, Koulen P. Plate reader-based cell viability assays for glioprotection using primary rat optic nerve head astrocytes. Exp Eye Res. 2015 Sep;138:159-66. doi: 10.1016/j.exer.2015.05.023.
- Kaja S, Payne AJ, Naumchuk Y, Levy D, Zaidi DH, Altman AM, Nawazish S, Ghuman JK, Gerdes BC, Moore MA, Koulen P. Plate reader-based cell viability assays for glioprotection using primary rat optic nerve head astrocytes. Exp Eye Res. 2015 Sep;138:159-66. doi: 10.1016/j.exer.2015.05.023.
LATEST NEWS
Refinement of the EAE Mouse Model
Novel model for corneal permeability in vitro testing through the combined use of collagen-based hydrogels with stratified corneal epithelial cells
A synthetic metalloporphyrin SOD mimetic protects corneal epithelial cells from oxidative stress-induced damage in vitro and in vivo
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