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Category: Neuroprotection / glioprotection

Primary dissociated retinal cells offer a rapid and efficient way to assay the neuroprotective effects of drug candidates on retinal ganglion cells. Primary acutely dissociated retinal cell culture is prepared from adult Wistar rats and subjected to trophic factor withdrawal.

Cell viability is assessed by double-label immunocytochemistry using specific antibodies for Thy1 and neurofilament (NFL).

SUMMARY

Species

Rats (Wistar)

In vitro Insult

Trophic factor withdrawal

Format

8 chamber well slides

Read-outs

Double label immunocytochemistry

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IMAGES

Thy1.1 and NFL immunoreactivities in primary acutely dissociated retinal cell culture

Two retinal ganglion cells in a field of view immunoreactive for Thy1.1 and NFL. Cells were exposed to a neuroprotective compound maintained under growth factor withdrawal conditions for 72 hr.

SELECTED PUBLICATIONS

  • Burroughs SL, Duncan RS, Rayudu P, Kandula P, Payne AJ, Clark JL, Koulen P, Kaja S. Plate reader-based assays for measuring cell viability, neuroprotection and calcium in primary neuronal cultures. J Neurosci Methods. 2012,203(1):141-145. doi: 10.1016/j.jneumeth.2011.09.007.

REFERENCES

  1. Kaja S, Duncan RS, Longoria S, Hilgenberg JD, Payne AJ, Desai NM, Parikh RA, Burroughs SL, Gregg EV, Goad DL, Koulen P. Novel mechanism of increased Ca2+ release following oxidative stress in neuronal cells involves type 2 inositol-1,4,5-trisphosphate receptors. Neuroscience. 2011,175:281-291. doi: 10.1016/j.neuroscience.2010.11.010.

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