Search the webpage

Category: Angiogenesis and Neovascularization
Animals used for research:   Rat  

Animal species

Streptozotocin (STZ) is a N-nitro derivative of glucosamine that is toxic to the insulin-producing pancreatic beta-cells. STZ-induced diabetic retinopathy model represents a popular and highly reproducible preclinical model to mimic type I diabetes mellitus in humans. However, this model mimics only very early phase of diabetic retinopathy without prominent vascular changes in the retina.

Indications: Type I Diabetes

SUMMARY

Animal species

Rats 

Method of induction

Injection of streptozotocin

Follow-up period

Up to 12 months

Route of compound administration

Topical (e.g. eye drops), intravitreal injection, systemic

Read-outs
  1. In vivo imaging:
    • fundoscopy;
    • fluorescein angiography;
    • optical coherence tomography (OCT);
    • vitreofluorophotometry.
  2. In vivo functional assessment
  3. Morphological assessment
  4. Molecular biology (ELISA, Western blotting, qPCR)
Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

Macroglial activation in STZ-induced rat retina (glial fibrillary acidic protein immunostaining)

The glial fibrillary acidic protein staining in the rat retinal wholemounts

SELECTED PUBLICATIONS

  1. Ragauskas S, Kielczewski E, Vance J, Kaja S, Kalesnykas G. In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model. J Vis Exp (2018). PubMed PMID: 29443029
  2. Kinnunen K, Heinonen SE, Kalesnykas G, Laidinen S, Uusitalo-Järvinen H, Uusitalo H, Ylä-Herttuala S. LDLR-/-ApoB100/100 mice with insulin-like growth factor II overexpression reveal a novel form of retinopathy with photoreceptor atrophy and altered morphology of the retina. Mol Vis. (2013). 19:1723-33. PubMed PMID: 23922490
  3. Viita H, Kinnunen K, Eriksson E, Lähteenvuo J, Babu M, Kalesnykas G, Heikura T, Laidinen S, Takalo T, Ylä-Herttuala S. Intravitreal adenoviral 15-lipoxygenase-1 gene transfer prevents vascular endothelial growth factor A-induced neovascularization in rabbit eyes. Hum Gene Ther (2009).1679-86. PubMed PMID: 19694557.
  4. Kinnunen K, Kalesnykas G, Mähönen AJ, Laidinen S, Holma L, Heikura T, Airenne K, Uusitalo H, Ylä-Herttuala S. Baculovirus is an efficient vector for the transduction of the eye: comparison of baculovirus- and adenovirus-mediated intravitreal vascular endothelial growth factor D gene transfer in the rabbit eye. J Gene Med (2009). 11:382-9. PubMed PMID: 19263462.

REFERENCES

  • Kern TS, Miller CM, Tang J, Du Y, Ball SL, Berti-Matera L. Comparison of three strains of diabetic rats with respect to the rate at which retinopathy and tactile allodynia develop. Mol Vis. 2010 Aug 15;16:1629-39. PubMed PMID: 20806092.

Leave a Comment

LATEST NEWS

Experimentica at BioJapan 2018

News
Experimentica Ltd. exhibits at BioJapan2018 partnering event in Pacifico Yokohama, Japan, on October 10 – October 12 ... Read More

Meet Experimentica Ltd. team at EVER 2018

News
Visit our booth to explore our state-of-the-art capabilities in preclinical research and our posters on diabetic retinopathy, glaucoma, dry and ... Read More

Subscribe to Our Newsletter!

We promise that no sales pitches will be attached to newsletter. And don't worry, we hate spammers too!