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Category: Neuroprotection
Animals used for research:  Mouse 

Mouse NAION model represents a popular method to induce acute retinal ischemia using Rose Bengal injection and consequent laser photocoagulation of optic nerve head area.

SUMMARY

Animal species

Mice

Method of induction

Rose Bengal injection (iv) and laser of optic nerve head area

Follow-up period

Typically 1-2 weeks

Route of compound administration

Topical (e.g. eye drops), intravitreal injections, systemic (i.v., i.p.), subcutaneous

Read-outs
  1. In vivo imaging (fluorescein angiography, optical coherence tomography)
  2. In vivo functional assessment: 
    • electroretinography 
  3. Morphological assessment:
    • optic nerve axon counts (semi-thin optic nerve sections)
    • routine histology (H&E staining for retinal sections)
    • immunohistochemistry (typically RGC marker/glial marker and counterstain in retinal wholemounts),
    • stereology of RGCs (retinal wholemounts) and optic nerve axons
  4. Molecular biology (ELISA, Western blotting, qPCR)
Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

Experimentica Ltd. uses state-of-the-art in vivo imaging methodologies, which allow the longitudinal evaluation of pathological changes

Ischemic retina (outlined in red, figure on the right) 3-day post-NAION induction. Retina from contralateral control eye (left) is intact.

Retinal wholemounts form control and NAION-induced eyes immunostained against RGC marker RBPMS (in red) and astrocytic marker GFAP (in green). DAPI was used for counterstain (in blue).

Total number of RBPMS-stained RGCs in control (49,014 ± 2,372, mean ± S.D.) and NAION (7,697 ± 4,015) mice.

SELECTED PUBLICATIONS

  1. Guo Y, Mehrabian Z, Bernstein SL. The Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy (rNAION). J Vis Exp. 2016 Nov 20;(117). doi: 10.3791/54504.

  2. Mathews MK, Guo Y, Langenberg P, Bernstein SL. Ciliary neurotrophic factor (CNTF)-mediated ganglion cell survival in a rodent model of non-arteritic anterior ischaemic optic neuropathy (NAION). Br J Ophthalmol. 2015 Jan;99(1):133-7. doi: 10.1136/bjophthalmol-2014-305969.

REFERENCES

  1. Ragauskas S, Ziniauskaite A, Kaja S, Kalesnykas G. Neuroprotection of retinal ganglion cells via NMDA-dependent mechanism in the mouse non-arteritic anterior ischemic optic neuropathy (NAION) model. ARVO 2018, programme #3204.

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