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Category: Neuroprotection
Animals used for research:  Mouse 

The detachment of photoreceptors from retinal pigment epithelium is evident in various diseases such as age-related macular degeneration (AMD), diabetic retinopathy (DR) or retinopathy of prematurity (ROP). Detached photoreceptors undergo cellular death, which subsequently leads to visual decline. Subretinal injection of sodium hyaluronate induces retinal detachment (RD) and photoreceptor cells death.1-3

Indications: Neuroprotection, age-related macular degeneration

SUMMARY

Animal species

Mice

Method of induction

Subretinal injection

Follow-up period

Up to 7 days

Route of compound administration

Topical (e.g. eye drops), subretinal, intravitreal, systemic

Read-outs
  1. In vivo imaging:
    • fundoscopy,
    • fluorescein angiography,
    • optical coherence tomography (OCT),
  2. In vivo functional assessment,
  3. Morphological assessment,
  4. Molecular biology (ELISA, Western blotting, qPCR).
Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

Experimentica Ltd. uses state-of-the-art in vivo imaging methodologies, which allow the longitudinal evaluation of pathological changes

Micrographs taken using SD-OCT at the level of optic nerve head (green line) before RD, immediately after RD and 7 days after RD. Right column indicates volume intensity projections of fundus. Subretinal injection of Poly (I:C) successfully caused retinal detachment as verified by SD-OCT (Ragauskas et al., ARVO 2016 poster).

A mouse eye sectioned at the site of retinal detachment (asterisk). H&E staining.

SELECTED PUBLICATIONS

  1. Anderson DH, Guerin CJ, Erickson PA, Stern WH, Fisher SK. Morphological recovery in the reattached retina. Invest. Ophthalmol. Vis. Sci. 1986;27:168–183.
  2. Nakazawa T, et al. Tumor Necrosis Factor-Mediates Photoreceptor Death in a Rodent Model of Retinal Detachment. Invest. Ophthalmol. Vis. Sci. 2011;52:1384–1391.
  3. Matsumoto H, Miller JW, Vavvas DG. Retinal detachment model in rodents by subretinal injection of sodium hyaluronate. J Vis Exp. 2013 Sep 11;(79). doi:10.3791/50660. 

REFERENCES

 

  1. Ragauskas S, Gruener S, Collin L, Kalesnykas G. Subretinal injection of poly(I:C) causes acute photoreceptor cell death in the mouse retinal detachment model. ARVO 2016 poster presentation.

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