Summary: Retinal detachment happens in AMD, Diabetic Retinopathy, and Retinopathy of Prematurity.
Model Description
The detachment of photoreceptors from retinal pigment epithelium is evident in various diseases such as age-related macular degeneration (AMD), diabetic retinopathy (DR) or retinopathy of prematurity (ROP). Detached photoreceptors undergo cellular death, which subsequently leads to visual decline. Subretinal injection of sodium hyaluronate induces retinal detachment (RD) and photoreceptor cells death.1-3
Animal species | Mice |
Method of induction | Subretinal injection |
Follow-up period | Up to 7 days |
Route of compound administration | Topical (e.g. eye drops), subretinal, intravitreal, systemic |
Read-outs | 1. In vivo imaging: – Fundoscopy, – Fluorescein angiography, – Optical coherence tomography (OCT), 2. In vivo functional assessment, 3. Morphological assessment, 4. Molecular biology (ELISA, Western blotting, qPCR). |
Outcomes and Read-Outs
In vivo imaging
Experimentica uses state-of-the-art in vivo imaging methodologies, which allow the longitudinal evaluation of pathological changes.
References
- Ragauskas S, Gruener S, Collin L, Kalesnykas G. Subretinal injection of poly(I:C) causes acute photoreceptor cell death in the mouse retinal detachment model. ARVO 2016 poster presentation.
- Anderson DH, Guerin CJ, Erickson PA, Stern WH, Fisher SK. Morphological recovery in the reattached retina. Invest. Ophthalmol. Vis. Sci. 1986;27:168–183.
- Nakazawa T, et al. Tumor Necrosis Factor-Mediates Photoreceptor Death in a Rodent Model of Retinal Detachment. Invest. Ophthalmol. Vis. Sci. 2011;52:1384–1391.
- Matsumoto H, Miller JW, Vavvas DG. Retinal detachment model in rodents by subretinal injection of sodium hyaluronate. J Vis Exp. 2013 Sep 11;(79). doi:10.3791/50660.