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Category: Angiogenesis and Neovascularization, Neuroprotection
Animals used for research:  Rabbit 

Age-related macular degeneration (AMD) is the leading cause of central vision loss for people age 50 and older. This vision loss makes it harder to do most of the day-to-day activities, like driving, cooking and reading. Sodium-iodide (NaIO3) induced retinal degeneration models have been widely used to study the cell death in AMD. Sodium iodate results in death of retinal pigmented epithelium (RPE) followed by a secondary death of photoreceptors, similar to what is observed in atrophic AMD (or geographic atrophy, GA).

SUMMARY

Animal species

Rabbit

Method of induction

Injection of sodium-iodide

Follow-up period

Typically up to 35 days

Route of compound administration

Intravitreal, topical, systemic

Read-outs

In vivo imaging: fluorescein angiography for damaged area quantification and optical coherence tomography for retinal thickness;

Functional assessment: rod bipolar cell function protection.

Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

Fig. 1. High-resolution imaging using fluorescein angiography (FA) and quantification of Area of Damage. A. FA images from untreated and treated (FAS inhibitor) sodium iodide (NaIO3- induced rabbits) on Day 3 after induction. FAS inhibitor treated eyes show decreased retinal damage area compared to untreated. The arrow points at the drug deposit in the vitreous. B. Area of damage, expressed in pixels (left y-axis) and percentage (right y-axis). Gray = untreated eyes; Blue= FAS inhibitor treated eyes.

Fig. 2. Protection of retinal thickness in NaIO3 induced rabbit model in eyes treated with high dose of FAS inhibitor.  Mean retinal thickness on day 0, 7, 14, 28 and 35 after NaIO3 induction  and FAS inhibitor treatment on Optical Coherence Tomography (OCT). Black= untreated; blue= low dose treated; Gray= high dose treated eyes.

Fig. 3. Protection of rod bipolar cell function in NaIO3 induced rabbit model in eyes treated with high dose of FAS inhibitor. Amplitude of b-wave on day 0, 7, 14, 28 and 35 after NaIO3 induction and FAS inhibitor treatment in flash electroretinograms. Black= untreated; blue= low dose treated; Gray= high dose treated eyes.

REFERENCES

Kocab AJ, Bacellar-Galdino M, Zacks DN, Jamison JA, Van De Goor J. Inhibition of Fas with ONL 1204 provides protection in a novel rabbit model of dry AMD. 2020. ARVO annual meeting. Poster presentation.