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Category: Angiogenesis and Neovascularization
Animals used for research:  Rabbit 

Vascular endothelial growth factor (VEGF) is up regulated in wet AMD and diabetic retinopathy. This up-regulation leads to abnormal retinal vascular growth and leakage that causes visual impairment. Intravitreal injection of VEGF causes a transient retinal leakage mimicking the disease.

At Experimentica, we have fully implemented and validated rabbit VEGF-induced retinal leakage model with a clinically relevant reference compound and the most comprehensive list of read-outs.

SUMMARY

Animal species

Rabbit

Method of induction

Injection of vascular endothelial growth factor (VEGF)

Follow-up period

Typically up to 14 days

Route of compound administration

Intravitreal, topical, systemic

Read-outs

In vivo imaging: qualitative grading of retinal leakage (fluorescein angiography);

Histology: post-mortem assessment of morphological changes both qualitatively and quantitatively.

Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

Fig. 1. High-resolution imaging using fluorescein angiography (FA). FA images from Vascular endothelial growth factor (VEGF) induced retinal leakage in rabbits are shown. Image in the top row show a control vasculature FA in a rabbit. Images in the second-row show day 3 images of  vascular leakage  in a VEGF –induced eye  treated with saline. Images in the third-row show day 3 images of  vascular leakage suppression in a representative VEGF-induced eye treated with Avastin®.

Fig. 2. Vascular Endothelial Growth Factor (VEGF) dosage range study for retinal leakage induction. A. Mean leakage scores from fluorescein angiography images graded 3 days post VEGF induction. B. Mean leakage scores from fluorescein angiography images graded 7 days post VEGF induction. * ANOVA (p<0.05).

REFERENCES

Donatas Neverauskas, DVM. In vivo imaging of VEGF-induced retinal neovascularization in Rabbits. Experimentica's webinar series, May 20th, 2020.