Primary human trabecular meshwork cells

Summary: A validated trabecular meshwork cells model for Glaucoma and custom in vitro studies.

Model Description

Experimentica Ltd. offers a validated human trabecular meshwork cells model for a variety of custom in vitro projects targeted at the trabecular meshwork. 

Species Primary Human trabecular meshwork cells 
In vitro insult Custom 
Format 96-well plate 
Read-outs Custom, e.g. actin fiber length, focal adhesions 

Outcomes and Read-Outs 

Imaging

Representative examples from primary human trabecular meshwork cells. 

Fig. 1. Effect of the rho kinase inhibitor netarsudil on actin fiber arrangement in primary human trabecular meshwork cells.
Fig. 1. Effect of the rho kinase inhibitor Netarsudil on actin fiber arrangement in primary human trabecular meshwork cells. Netarsudil (1.2 µM) results in a significant reduction of actin fiber length as assessed by labeling with fluorescently-labelled phalloidin (Actin GreenTM 488 ReadyProbesTM, Thermo Fisher). 
Fig. 2. Effect of the rho kinase inhibitor netarsudil on focal adhesions in primary human trabecular meshwork cells.
Fig. 2. Effect of the rho kinase inhibitor Netarsudil on focal adhesions in primary human trabecular meshwork cells. Netarsudil (1.2 µM) results in a significant reduction in the number of focal adhesions, as quantified using anti-paxillin antibody (clone 5H11, EMD Millipore). 

Authors' picture