Summary: A rapid, efficient way to assess the neuroprotective effects of drug candidates on retinal ganglion cells.
Primary dissociated retinal cells offer a rapid and efficient way to assay the neuroprotective effects of drug candidates on retinal ganglion cells. Primary acutely dissociated retinal cell culture is prepared from adult Wistar rats and subjected to trophic factor withdrawal.
Cell viability is assessed by double-label immunocytochemistry using specific antibodies for Thy1 and neurofilament (NFL).
|Species||Wistar Rat primary dissociated retinal cells|
|In vitro insult||Trophic factor withdrawal|
|Format||8 chamber well slides|
|Read-outs||Double label immunocytochemistry|
Outcomes and Read-Outs
- Kaja S, Duncan RS, Longoria S, Hilgenberg JD, Payne AJ, Desai NM, Parikh RA, Burroughs SL, Gregg EV, Goad DL, Koulen P. Novel mechanism of increased Ca2+ release following oxidative stress in neuronal cells involves type 2 inositol-1,4,5-trisphosphate receptors. Neuroscience. 2011,175:281-291. doi: 10.1016/j.neuroscience.2010.11.010.
- Burroughs SL, Duncan RS, Rayudu P, Kandula P, Payne AJ, Clark JL, Koulen P, Kaja S. Plate reader-based assays for measuring cell viability, neuroprotection and calcium in primary neuronal cultures. J Neurosci Methods. 2012,203(1):141-145. doi: 10.1016/j.jneumeth.2011.09.007.