Primary retinal cell culture

Summary: A rapid, efficient way to assess the neuroprotective effects of drug candidates on retinal ganglion cells.

Model Description

Primary dissociated retinal cells offer a rapid and efficient way to assay the neuroprotective effects of drug candidates on retinal ganglion cells. Primary acutely dissociated retinal cell culture is prepared from adult Wistar rats and subjected to trophic factor withdrawal. 

Cell viability is assessed by double-label immunocytochemistry using specific antibodies for Thy1 and neurofilament (NFL). 

Species Wistar Rat primary dissociated retinal cells 
In vitro insult Trophic factor withdrawal 
Format 8 chamber well slides 
Read-outs Double label immunocytochemistry  

Outcomes and Read-Outs 

Immunocytochemistry 

Fig. 1. Two retinal ganglion cells in a field of view immunoreactive for Thy1.1 and NFL.
Fig. 1. Two retinal ganglion cells in a field of view immunoreactive for Thy1.1 and NFL. Cells were exposed to a neuroprotective compound maintained under growth factor withdrawal conditions for 72 hours. 

Authors' picture