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Category: Neuroprotection
Animals used for research:  Rat 

The rodent blue light damage (BLD) model is a well-established model for retinal degeneration, oxidative stress and neuroinflammatory activity. BLD is a multi-faceted process affecting chromophores that absorb blue light, such as cytochrome oxidase and rhodopsin. The BLD model is a quick inducible method for causing photo-oxidative damage that is primarily localized to the outer retina, including bipolar, photoreceptor and RPE cell layers. Because cell death is apoptotic rather than necrotic, the BLD model mirrors retinal degeneration in human diseases including AMD, retinitis pigmentosa (RP), and retinal detachment. Acute BLD typically results in geographically irregular apoptosis and retinal atrophy, which is similar to atrophy seen in the terminal stage of dry AMD.

At Experimentica, we have fully implemented and validated the rat BLD model with clinically relevant reference compounds and the most comprehensive list of read-outs.

SUMMARY

Animal species

Rat

Method of induction

Blue light

Follow-up period

Typically up to 7 days

Route of compound administration

Intravitreal, topical, systemic

Read-outs

Flash electroretinography (fERG): functional deficits are observed both in a-wave and b-wave amplitudes in 6- and 8-hour exposure to blue light;

Histology: quantitative assessment of retinal thickness

Please contact us, if you would like to receive more information: info@experimentica.com

IMAGES

Fig. 1 . Flash electroretinograms (ERG) in the Blue light damage (BLD) rat model. Retinal damage is dependent on light exposure time. ERGs were performed 5 days after light exposure.

Fig. 2. Maximum response amplitude on flash electroretinograms (ERG) in the Blue light damage (BLD) rat model following 0.5% Betaxon topical ocular treatment. Retinal function is protected in Betaxon treated eyes.

Fig. 3. Retinal Histology sections of Blue light damage (BLD) rat model following 0.5% Betaxon topical ocular treatment. Retinal damage is  observed on vehicle treated eyes.