Assessment of anti-angiogenic potential (HUVEC tube formation assay)

Summary: A validated model to test the anti-angiogenic potential of a drug.

Model Description

This model is used to test the anti-angiogenic potential of an active pharmaceutical ingredient (API) in human umbilical vein endothelial cells (HUVEC) in vitro. 

HUVECs are grown on 15-well µ-Slide Angiogenesis (Ibidi GmbH, Germany). The cells can be exposed to study compounds at different concentrations for 6h. 

Species Human umbilical vein endothelial cells (HUVEC) 
Induction Angiogenin and VEGF 
Positive control Sulforaphane 
Analysis ImageJ or Wimasis 
Indicator and Read-outs Total tube length Total number of segments 

Outcomes and Read-Outs 

Imaging

Fig. 1. Image analysis of the HUVEC tube formation assay with basal media and growth media controls, angiogenic inducer (angiogenin) and inhibitor for proliferative activity of endothelial cells (sulforaphane).
Fig. 1. Image analysis of the HUVEC tube formation assay with basal media and growth media controls, angiogenic inducer (angiogenin) and inhibitor for proliferative activity of endothelial cells (sulforaphane). 
Fig. 2. Total tube length and Total number of segments in the HUVEC tube formation assay.
Fig. 2. Total tube length and Total number of segments in the HUVEC tube formation assay. 

Authors' picture