Summary: An assessment and prediction tool of a drug-induced toxicity on human retinoblastoma cells.
An assessment and prediction of study compound-induced toxicity on the human retinoblastoma cells (WERI-Rb-1) in vitro.
|Cells||WERI-Rb-1 cells1-2 (RIKEN, Japan)|
|Viability assessment||WERI-Rb-1 cells are grown on 96-well plates. The cells can be exposed to testing molecules or formulations for selected times (e.g. 4h, 24h). Cytotoxicity is measured using resazurin reduction assay3. A cell permeable redox indicator resazurin (7-Hydroxy-3H-phenoxazin-3-one-10-oxide) is used to measure oxidation-reduction reactions in viable cells. The non-fluorescent resazurin is irreversibly reduced by metabolically active cells to highly fluorescent metabolite resorufin (7-Hydroxy-3H-phenoxazin-3-one). Resorufin formation is measured by using Cytation 3 multi-mode reader (BioTek Instruments, Winooski, VT, USA). The quantity of resorufin produced is proportional to the number of viable cells.|
|Positive control||tert-butyl hydroperoxide (tBHP) at concentrations 0-30 mM|
|Read-outs||1. Cell viability (%)|
2. IC50 value (50% cell viability) for positive control
3. IC50 value is calculated for study compound if applicable
Outcomes and Read-Outs
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