Summary: An assessment and prediction of drug-induced neuroprotection and toxicity in rat primary cortical neurons.
An assessment and prediction of study compound-induced neuroprotection or toxicity on the rat primary cortical neurons in vitro.
|Cells||Rat primary cortical neurons|
|Cell viability assessment||Rat cortical neurons are grown on 96-well plates. The cells can be exposed to testing molecules or formulations for selected times (e.g. 4h, 24h). Cytotoxicity is measured using resazurin reduction assay. A cell permeable redox indicator resazurin (7-Hydroxy-3H-phenoxazin-3-one-10-oxide) is used to measure oxidation-reduction reactions in viable cells. The non-fluorescent resazurin is irreversibly reduced by metabolically active cells to highly fluorescent metabolite resorufin (7-Hydroxy-3H-phenoxazin-3-one). Resorufin formation is measured by using Cytation 3 multi-mode reader (BioTek Instruments, Winooski, VT, USA). The quantity of resorufin produced is proportional to the number of viable cells.|
|Oxidative stress inducer||tert-butyl hydroperoxide (tBHP) at concentrations 0-40 mM|
|Positive control||(±)-6-Hydroxy-2,5,7,8- tetramethylchromane-2-carboxylic acid (Trolox) 1000 mM|
|Read-outs||Cell viability (%)|
IC50 value (50% cell viability) for positive control
IC50 value is calculated for study compound if applicable
Outcomes and Read-Outs
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