Ex Vivo Retinal Wholemount

Summary: Organotypic retinal tissue ex vivo provides a simplified model to study retinal cell degeneration and screen promising drugs.

Model Description

Organotypic retinal tissue ex vivo provides a simplified model to study retinal cell degeneration and screen promising therapeutics under strictly controlled environmental conditions1 up to 14 days in vitro2

Animal speciesMice, Rats
Follow-up periodUp to 14 days
Route of compound administrationIn the serum-free media
Read-outs1. Ex vivo functional assessment;
2. Morphological assessment;
3. Molecular biology (ELISA, Western blotting, qPCR).

Outcomes and Read-Outs 

Imaging

Fig. 1. Immunostaining of GFAP (green, left) and melanopsin (red, middle) with nuclear counterstain (blue, DAPI). Hematoxylin and eosin staining of ex vivo retinal explant (right).
Fig. 1. Immunostaining of GFAP (green, left) and melanopsin (red, middle) with nuclear counterstain (blue, DAPI). Hematoxylin and eosin staining of ex vivo retinal explant (right). 

Authors' picture