Summary: Organotypic retinal tissue ex vivo provides a simplified model to study retinal cell degeneration and screen promising drugs.
Model Description
Organotypic retinal tissue ex vivo provides a simplified model to study retinal cell degeneration and screen promising therapeutics under strictly controlled environmental conditions1 up to 14 days in vitro2.
Animal species | Mice, Rats |
Follow-up period | Up to 14 days |
Route of compound administration | In the serum-free media |
Read-outs | 1. Ex vivo functional assessment; 2. Morphological assessment; 3. Molecular biology (ELISA, Western blotting, qPCR). |
Outcomes and Read-Outs
Imaging
References
- Nymark S, Ragauskas S, Savolainen V, Holme A, Hyttinen JAK, Kalesnykas G. Morphological and functional characterization of ex vivo retinal explants from adult mice. ARVO 2014 poster. Investigative Ophthalmology & Visual Science, 2014, April, Vol. 55, 2377.
- Bull ND, Johnson TV, Welsapar G, DeKorver NW, Tomarev SI, Martin KR. Use of an adult rat retinal explant model for screening of potential retinal ganglion cell neuroprotective therapies. Invest Ophthalmol Vis Sci. 2011 May 17;52(6):3309-20. doi:10.1167/iovs.10-6873.
- Alarautalahti V, Ragauskas S, Hakkarainen JJ, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S. Viability of Mouse Retinal Explant Cultures Assessed by Preservation of Functionality and Morphology. Investigative Ophthalmology & Visual Science 2019;60(6):1914-1927. doi: 10.1167/iovs.18-25156.