Laser-induced Geographic Atrophy

Summary: The mouse laser-induced geographic atrophy (GA) model resembles human early phase GA-like pathology in mice.

Model Description

The mouse laser-induced geographic atrophy (GA) model is based on the publication by Ibbett and colleagues (2019).

Briefly, early GA-like features are induced using a 810 nm diode laser unilaterally. The contralateral eye serves as control. The presence and progression of GA-like changes are monitored using in vivo imaging (fundus photography (FP) and spectral-domain optical coherence tomography (SD-OCT)) up to 60 days, starting at baseline (prior the lasering), and then weekly post-lasering. At the end of the study period, mice are sacrificed, and whole eyes are collected for further histological and immunohistochemical assessment.

The model resembles human early phase GA-like pathology in mice.

Animal speciesMice
Method of induction810 nm diode laser
Follow-up periodUp to 60 days, but typically 28 days
Route of compound administrationTopical (e.g. eye drops), intravitreal or subretinal injections, systemic
Read-outs1. In vivo imaging
– Fundus photography (FP)
– Spectral domain-ocular coherence tomography (SD-OCT)
2. Morphological assessment (histology, immunohistochemistry, electron microscopy, stereology)

Outcomes and Read-Outs

In vivo imaging

Fig. 1. In vivo imaging using fundoscopy at different timepoints post-lasering.
Fig. 2. Spectral-domain optical coherence tomography (SD-OCT) from lasered sites.

Histology/Morphometry

Fig. 3. Histology (H&E staining) and morphological evaluation of lasered sites 28 days after the lasering.
Fig. 4. Immunostaining of vimentin (green) at the site of lesion. DAPI was used as counterstain (in blue).
Fig. 5. Immunostaining of RPE tight junctions (in red) at the site of lasering. DAPI was used as counterstain (in blue).

Authors' picture