Summary: The mouse laser-induced geographic atrophy (GA) model resembles human early phase GA-like pathology in mice.
The mouse laser-induced geographic atrophy (GA) model is based on the publication by Ibbett and colleagues (2019).
Briefly, early GA-like features are induced using a 810 nm diode laser unilaterally. The contralateral eye serves as control. The presence and progression of GA-like changes are monitored using in vivo imaging (fundus photography (FP) and spectral-domain optical coherence tomography (SD-OCT)) up to 60 days, starting at baseline (prior the lasering), and then weekly post-lasering. At the end of the study period, mice are sacrificed, and whole eyes are collected for further histological and immunohistochemical assessment.
The model resembles human early phase GA-like pathology in mice.
|Method of induction||810 nm diode laser|
|Follow-up period||Up to 60 days, but typically 28 days|
|Route of compound administration||Topical (e.g. eye drops), intravitreal or subretinal injections, systemic|
|Read-outs||1. In vivo imaging|
– Fundus photography (FP)
– Spectral domain-ocular coherence tomography (SD-OCT)
2. Morphological assessment (histology, immunohistochemistry, electron microscopy, stereology)