Summary: A validated, standardized screening method for glioprotective drug candidates using primary adult rat ONHAs.
Optic nerve head remodeling is generally considered the earliest manifestation of the glaucomatous triad, resulting in the subsequent damage to the axons of the optic nerve and ultimately retinal ganglion cells death. Optic nerve head astrocytes (ONHAs) are the primary cell type in the optic nerve head, where they provide both structural and nutrient support to the optic nerve. Activation of ONHAs, also referred to reactive astrocytosis or astrogliosis, represents the underlying physiological mechanism of optic nerve head remodeling.
Targeting reactive astrocytosis (glioprotection) has recently gained a strong interest in the development of novel pharmaceutical strategies for glaucoma that can supplement IOP-lowering agents.
Experimentica Ltd. offers a validated and standardized screening method for glioprotective drug candidates using primary adult rat ONHAs.
|Species||Rats (Brown Norway)|
|In vitro insult||Oxidative stress (tert-butylhydroperoxide, tBHP)|
|Format||96-well plate, tBHP dose response, 8 replicates per concentration|
|Read-outs||Cell viability assays (e.g. MTT, lactate dehydrogenase)|
Outcomes and Read-Outs
Representative examples from primary rat ONHAs