Summary: The up-regulation of VEGF leads to abnormal retinal vascular growth and leakage that causes visual impairment. Intravitreal injection of VEGF causes a transient retinal leakage mimicking the like of wet AMD and diabetic retinopathy.
Vascular endothelial growth factor (VEGF) is up regulated in wet AMD and diabetic retinopathy. This up-regulation leads to abnormal retinal vascular growth and leakage that causes visual impairment. Intravitreal injection of VEGF causes a transient retinal leakage mimicking the disease.
At Experimentica, we have fully implemented and validated rabbit VEGF-induced retinal leakage model with a clinically relevant reference compound and the most comprehensive list of read-outs.
|Method of induction||Injection of vascular endothelial growth factor (VEGF)|
|Follow-up period||Typically up to 14 days|
|Route of compound administration||Intravitreal, topical, systemic|
|Read-outs||1. In vivo imaging:|
– Qualitative grading of retinal leakage (fluorescein angiography);
– Post-mortem assessment of morphological changes both qualitatively and quantitatively.
Outcomes and Read-Outs
In vivo imaging
Fluorescein angiography (rabbit; Heideleberg Spectralis). Retinal vascular leakage and leakage suppression can be imaged after VEGF induction and treatment
Dose range VEGF study showing the optimal dosage for retinal leakage.
- Neverauskas D. In vivo imaging of VEGF-induced retinal neovascularization in Rabbits. Experimentica webinar series, May 20th, 2020.