Disease models
Experimental Autoimmune Encephalomyelitis
Experimental Autoimmune Encephalomyelitis (EAE) is the most commonly used model for the preclinical development of drug candidates for treatment of multiple sclerosis1 (MS) and associated optic neuritis2.
The model is characterized by pathogenesis of autoimmunity, CNS inflammation, and demyelination. These pathologies lead to symptoms of paralysis and visual impairment. Thus, the EAE model mimics the phenotype reminiscent of MS with a well-defined onset of development, as well as aspects of the optic neuritis.
Technical details
– Emulsion containing MOG35-55 peptide and inactivated mycobacterium tuberculosis in complete Freuds adjuvant (CFA)
– Pertussis toxin (PTX)
– Retinal thickness assessment with SD-OCT imaging
– Assessment of visual function by electroretinogram, oscillatory potentials, photopic negative response, and/or visually evoked potentials
– Infiltration of optic nerve and
– Assessment of infiltration and demyelination
– Quantification of retinal ganglion cells
Scientific excellence in every model
A well-established phenotype for modelling multiple sclerosis
Experienced and fine-tuned behavioural scoring of clinical symptoms
Customised in vivo functional assessment of different aspects of vision
References
- Huntemann N, Vogelsang A, Groeneweg L, Willison A, Herrmann A M, Meuth S G, Eichler S (2022). An optimized and validated protocol for inducing chronic experimental autoimmune encephalomyelitis in C57BL/6J mice. Journal of Neuroscience Methods. 367
- Manogaran P, Walker-Egger C, Samardzija M, Waschkies C, Grimm C, Rudin M, Schippling S. Exploring experimental autoimmune optic neuritis using multimodal imaging. NeuroImage 175, 327-339
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