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Category: Cytotoxicity

An assessment and prediction of study compound-induced toxicity on the human retinoblastoma cells (WERI-Rb-1) in vitro.

SUMMARY

Cells

WERI-Rb-1 cells1-2 (RIKEN, Japan)

Exposure

Cells are exposed to study compounds at different concentrations for selected times (e.g. 4h, 24h)

Positive control

tert-butyl hydroperoxide (tBHP) at concentrations 0-30 mM

Indicator and detection

Indicator: resazurin (7-Hydroxy-3H-phenoxazin-3-one-10-oxide) dye is irreversibly reduced to highly fluorescent metabolite resorufin (7-Hydroxy-3H-phenoxazin-3-one);

 

Detection: after 2 h exposure to resazurin, the amount of resorufin is measured at 560 nm (ex), 590 nm (em) by using Cytation 3 multi-mode reader (BioTek Instruments, Winooski, VT, USA).

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IMAGES

Representative examples of assays on WERI-Rb-1 cells

Typical morphology of WERI-Rb-1 cells showing rosettes (R) and chain (C) formation. Scale bar 100 µm.

Characteristic grape-like glusters of WERI-Rb-1 cells at higher cell density. Scale bar 100 µm (left) and 200 µm (right).

SELECTED PUBLICATIONS

  • Campbell M, Chader GJ. Retinoblastoma cells in tissue culture. Ophthalmic Paediatr Genet. 1988 Nov;9(3):171-99. Review. PubMed PMID: 3068600.

REFERENCES

  1. McFall RC, Sery TW, Makadon M. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 1977,37(4):1003-1010.
  2. McFall RC, Nagy RM, Nagle BT, McGreevy LM. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 1978,38(9):2827-2835.

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