Neuroscience 2022 – Standardization of white and blue light-induced retinal phototoxicity models in pigmented mice

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By Experimentica on November 10, 2022

Summary: Marianna Bacellar-Galdino, DVM, PhD, Director of Veterinary Ophthalmology at Experimentica will present at SfN’s Neuroscience 2022.


Retinal phototoxicity induced by blue or white light is a well-established model to mimic aspects of dry age-related macular degeneration (AMD) and geographic atrophy. Induction of this model is usually performed in albino rodents lacking ocular pigmentation, thereby facilitating absorbance of light by photoreceptors and retinal pigment epithelium cells. Here we evaluated feasibility of induction of white and blue light damage in pigmented mice.

Our data revealed similar levels of retinal degeneration between pigmented and non-pigmented mice, providing feasibility for the use of pigmented mice in white and blue damage studies.

We provide a standardized light insult protocol that can be readily transferred to transgenic mouse strains that are maintained on pigmented genetic backgrounds.

Key takeaway:

  • Blue light and white light can elicit significant retinal phototoxicity in pigmented mice
  • Level of retinal damage is similar between pigmented and non-pigmented mice
  • Our standardized protocol can be readily transferred to transgenic pigmented mouse strains

Authors:   Marianna Bacellar-Galdino | Nate Pappenhagen | Simon Kaja | Jeff Jamison

Attend the poster presentation:

November 16, 2022, 4:00-5:00pm Pacific
San Diego Convention Center, Poster 707.16

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