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animal welfare mice with anti-skid mattress and paper shavings

Refinement of the EAE Mouse Model

Experimental Autoimmune Encephalomyelitis (EAE) is used to study Multiple Sclerosis (MS) a chronic neuroinflammatory demyelinating disorder of the CNS. EAE induces a T cell and mononuclear cell inflammation, which leads to a progressive hind-limb paralysis (Robinson et al., 2014). Due to the severity of EAE, the welfare of animals should be taken into account through the planning of humane endpoints (HEP), providing appropriate perioperative care and minimizing stress factors. Several refinement (3R’s) (W.M.S. Russell and R.L. Burch 1959) methods were tested in order to minimize the number of animals euthanized due to HEP ... Read More
Novel model for corneal permeability in vitro testing through the combined use of collagen-based hydrogels with stratified corneal epithelial cells

Novel model for corneal permeability in vitro testing through the combined use of collagen-based hydrogels with stratified corneal epithelial cells

The cornea is critical for penetration of topically applied drugs into the eye. Assessment of the permeability properties of drug candidates across the cornea is a pillar stone of early drug development for topical ocular drugs. Nonetheless, permeability tests using animal corneas are costly and time consuming, while experiments using cell-based systems might often overestimate permeability due to the lack of modeling the corneal stroma. The purpose of this study was to develop an in vitro model for reliable permeability testing based on the combined use of collagen-based hydrogels and stratified human epithelial cells (HCE-T) ... Read More
histology, immune cell infiltration, lacrimal glands, conjunctival goblet cells

A synthetic metalloporphyrin SOD mimetic protects corneal epithelial cells from oxidative stress-induced damage in vitro and in vivo

Keratoconjunctivitis sicca (KCS) is a multifactorial disease of the lacrimal glands and ocular surface1. Pathological findings in patients with KCS includes a reduction in goblet cells and the presence of inflammatory cell infiltrates in the lacrimal glands2. KCS usually involves tear hyperosmolarity and ocular surface inflammation. Increased tissue levels of reactive oxygen species (ROS) and the ensuing oxidative stress are common hallmarks KCS and thought to contribute to the associated ocular surface damage. Superoxide dismutases (SODs) are enzymes which are part of antioxidant system that protects against ROS through dismutation of superoxide to produce hydrogen peroxide2,3. The aim of this study was to determine the efficacy of Mn-TM-2-PyP (metalloporphyrin SOD mimetic) against oxidative-stress induced damage in corneal epithelial cells in in vitro, and in the SiccaSystemTM in vivo model ... Read More
SD-OCT

Bright light damage (BLD) model

Retinal damage induced by bright light (BLE) in laboratory rodents has remained one of the most widely used models mimicking human retinal degenerations such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD) for more than five decades. The model is induced by exposing rodents to bright light (2.500-10.000 lux). The BLE induces the apoptosis of photoreceptors and retinal pigment epithelium1-3. Previous studies have shown that the total retinal thickness decreases with aging, as seen in 7 vs 3.5 month-old mice. Moreover, the BLE exacerbates the retinal damage in aged mice when compared to young mice. Purpose The purpose of this study was to determine whether age, or lifetime exposure to light, is the primary determinant of photoreceptor viability in the BLE model ... Read More

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LATEST NEWS

Refinement of the EAE Mouse Model

Experimental Autoimmune Encephalomyelitis (EAE) is used to study Multiple Sclerosis (MS) a chronic neuroinflammatory demyelinating disorder of the CNS. EAE ... Read More

Novel model for corneal permeability in vitro testing through the combined use of collagen-based hydrogels with stratified corneal epithelial cells

The cornea is critical for penetration of topically applied drugs into the eye. Assessment of the permeability properties of drug ... Read More

A synthetic metalloporphyrin SOD mimetic protects corneal epithelial cells from oxidative stress-induced damage in vitro and in vivo

Keratoconjunctivitis sicca (KCS) is a multifactorial disease of the lacrimal glands and ocular surface1. Pathological findings in patients with KCS ... Read More

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